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pn 1000268 mom basic immunodetection kit vector laboratories cat  (Vector Laboratories)


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    Structured Review

    Vector Laboratories pn 1000268 mom basic immunodetection kit vector laboratories cat
    Pn 1000268 Mom Basic Immunodetection Kit Vector Laboratories Cat, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 2773 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pn+1000268+mom+basic+immunodetection+kit+vector+laboratories+cat/Mouse+on+Mouse+(M%2EO%2EM%2E+)+Basic+Kit/pm39353433-280-187-192
    Average 96 stars, based on 2773 article reviews
    pn 1000268 mom basic immunodetection kit vector laboratories cat - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Recombinant:

    Article Title: Alzheimer's disease-linked risk alleles elevate microglial cGAS-associated senescence and neurodegeneration in a tauopathy model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Sudan black B, fat soluble diazo dye Abcam Cat. # ab146284 Trypsin Gibco Cat. # 15090-45 DNase I Millipore Sigma Cat. # 260913 DMEM Corning Cat. # 10-017-CV Fetal bovine serum Gibco Cat. # 26140-079 Penicillin-streptomycin Gibco Cat. # 15070063 Poly-D-lysine Thermo Fisher Scientific Cat. # A389401 Recombinant mouse GM-CSF, carrier free R&D Systems Cat. # 415-ML-020-CF 0N4R tau fibrils Sue-Ann Mok, University of Alberta N/A RIPA lysis and extraction buffer Thermo Fisher Scientific Cat. # 89901 Halt phosphatase inhibitor cocktail Thermo Fisher Scientific Cat. # 78420 cOmplete protease inhibitor cocktail Roche Cat. # 11697498001 NuPAGE MES SDS running buffer Invitrogen Cat. # NP0002 Clarity Western ECL substrate Bio-Rad Cat. # 1705060 Calcein AM Invitrogen Cat. # C3099 Etoposide Sigma-Aldrich E1383 BAI1 (BAX channel blocker) Sigma-Aldrich96 Cat. # 196805 TDI6570 (cGAS inhibitor) Synthesized by Gan Lab at Wuxi AppTec (Tianjin) Co., Ltd.97 N/A Dimethyl sulfoxide (DMSO) MP Biomedicals Cat. # 02196055 Critical commercial assays PureLink RNA Mini Kit Invitrogen Cat. # 12183018A Nuclei Isolation Kit: Nuclei PURE Prep Sigma-Aldrich Cat. # NUC201-1KT Chromium Single Cell 30 Reagent Kit 10x Genomics Cat. # PN-1000268 MOM Basic Immunodetection Kit Vector Laboratories Cat. # BMK-2202 Quick-RNA MicroPrep Kit Zymo Research Cat. # R1051 BCA protein assay kit Pierce Cat. # 23225 ProcartaPlex Mouse Chemokine Panel 1, 9plex kit Themo Fisher Scientific Cat. # EPX090-20821-901 Mouse IFN-beta ELISA kit, high sensitivity PBL Assay Science Cat. # 42410 BrdU cell proliferation ELISA kit Abcam Cat. # ab126556 Deposited data Original western blot images This paper Mendeley Data: https://doi.org/ 10.17632/nw7sbsh56p.1 Raw and processed bulk RNA-sequencing data This paper GEO: GSE254103 Raw and processed single-nuclei RNA-sequencing data This paper GEO: GSE254104 Raw and processed proteomics data This paper PRIDE: PXD053544 Experimental models: Organisms/strains Prnp-MAPT*P301S (B6/C3) The Jackson Laboratory RRID:IMSR_JAX:008169 C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:000664 Human APOE3 or APOE4 (C57BL/6J) Sullivan et al.,55 Duke University N/A Human TREM2-CV (C57BL/6J) This paper N/A Human TREM2-R47H (C57BL/6J) Gan et al.,34 Weill Cornell Medicine N/A Software and algorithms Original code This paper Zenodo: https://doi.org/10.5281/ zenodo.13642999 R 4.2.3 The R project https://www.r-project.org/ (Continued on next page) e2 Neuron 112, 1–20.e1–e8, December 4, 2024

    Lysis:

    Article Title: Alzheimer's disease-linked risk alleles elevate microglial cGAS-associated senescence and neurodegeneration in a tauopathy model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Sudan black B, fat soluble diazo dye Abcam Cat. # ab146284 Trypsin Gibco Cat. # 15090-45 DNase I Millipore Sigma Cat. # 260913 DMEM Corning Cat. # 10-017-CV Fetal bovine serum Gibco Cat. # 26140-079 Penicillin-streptomycin Gibco Cat. # 15070063 Poly-D-lysine Thermo Fisher Scientific Cat. # A389401 Recombinant mouse GM-CSF, carrier free R&D Systems Cat. # 415-ML-020-CF 0N4R tau fibrils Sue-Ann Mok, University of Alberta N/A RIPA lysis and extraction buffer Thermo Fisher Scientific Cat. # 89901 Halt phosphatase inhibitor cocktail Thermo Fisher Scientific Cat. # 78420 cOmplete protease inhibitor cocktail Roche Cat. # 11697498001 NuPAGE MES SDS running buffer Invitrogen Cat. # NP0002 Clarity Western ECL substrate Bio-Rad Cat. # 1705060 Calcein AM Invitrogen Cat. # C3099 Etoposide Sigma-Aldrich E1383 BAI1 (BAX channel blocker) Sigma-Aldrich96 Cat. # 196805 TDI6570 (cGAS inhibitor) Synthesized by Gan Lab at Wuxi AppTec (Tianjin) Co., Ltd.97 N/A Dimethyl sulfoxide (DMSO) MP Biomedicals Cat. # 02196055 Critical commercial assays PureLink RNA Mini Kit Invitrogen Cat. # 12183018A Nuclei Isolation Kit: Nuclei PURE Prep Sigma-Aldrich Cat. # NUC201-1KT Chromium Single Cell 30 Reagent Kit 10x Genomics Cat. # PN-1000268 MOM Basic Immunodetection Kit Vector Laboratories Cat. # BMK-2202 Quick-RNA MicroPrep Kit Zymo Research Cat. # R1051 BCA protein assay kit Pierce Cat. # 23225 ProcartaPlex Mouse Chemokine Panel 1, 9plex kit Themo Fisher Scientific Cat. # EPX090-20821-901 Mouse IFN-beta ELISA kit, high sensitivity PBL Assay Science Cat. # 42410 BrdU cell proliferation ELISA kit Abcam Cat. # ab126556 Deposited data Original western blot images This paper Mendeley Data: https://doi.org/ 10.17632/nw7sbsh56p.1 Raw and processed bulk RNA-sequencing data This paper GEO: GSE254103 Raw and processed single-nuclei RNA-sequencing data This paper GEO: GSE254104 Raw and processed proteomics data This paper PRIDE: PXD053544 Experimental models: Organisms/strains Prnp-MAPT*P301S (B6/C3) The Jackson Laboratory RRID:IMSR_JAX:008169 C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:000664 Human APOE3 or APOE4 (C57BL/6J) Sullivan et al.,55 Duke University N/A Human TREM2-CV (C57BL/6J) This paper N/A Human TREM2-R47H (C57BL/6J) Gan et al.,34 Weill Cornell Medicine N/A Software and algorithms Original code This paper Zenodo: https://doi.org/10.5281/ zenodo.13642999 R 4.2.3 The R project https://www.r-project.org/ (Continued on next page) e2 Neuron 112, 1–20.e1–e8, December 4, 2024

    Extraction:

    Article Title: Alzheimer's disease-linked risk alleles elevate microglial cGAS-associated senescence and neurodegeneration in a tauopathy model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Sudan black B, fat soluble diazo dye Abcam Cat. # ab146284 Trypsin Gibco Cat. # 15090-45 DNase I Millipore Sigma Cat. # 260913 DMEM Corning Cat. # 10-017-CV Fetal bovine serum Gibco Cat. # 26140-079 Penicillin-streptomycin Gibco Cat. # 15070063 Poly-D-lysine Thermo Fisher Scientific Cat. # A389401 Recombinant mouse GM-CSF, carrier free R&D Systems Cat. # 415-ML-020-CF 0N4R tau fibrils Sue-Ann Mok, University of Alberta N/A RIPA lysis and extraction buffer Thermo Fisher Scientific Cat. # 89901 Halt phosphatase inhibitor cocktail Thermo Fisher Scientific Cat. # 78420 cOmplete protease inhibitor cocktail Roche Cat. # 11697498001 NuPAGE MES SDS running buffer Invitrogen Cat. # NP0002 Clarity Western ECL substrate Bio-Rad Cat. # 1705060 Calcein AM Invitrogen Cat. # C3099 Etoposide Sigma-Aldrich E1383 BAI1 (BAX channel blocker) Sigma-Aldrich96 Cat. # 196805 TDI6570 (cGAS inhibitor) Synthesized by Gan Lab at Wuxi AppTec (Tianjin) Co., Ltd.97 N/A Dimethyl sulfoxide (DMSO) MP Biomedicals Cat. # 02196055 Critical commercial assays PureLink RNA Mini Kit Invitrogen Cat. # 12183018A Nuclei Isolation Kit: Nuclei PURE Prep Sigma-Aldrich Cat. # NUC201-1KT Chromium Single Cell 30 Reagent Kit 10x Genomics Cat. # PN-1000268 MOM Basic Immunodetection Kit Vector Laboratories Cat. # BMK-2202 Quick-RNA MicroPrep Kit Zymo Research Cat. # R1051 BCA protein assay kit Pierce Cat. # 23225 ProcartaPlex Mouse Chemokine Panel 1, 9plex kit Themo Fisher Scientific Cat. # EPX090-20821-901 Mouse IFN-beta ELISA kit, high sensitivity PBL Assay Science Cat. # 42410 BrdU cell proliferation ELISA kit Abcam Cat. # ab126556 Deposited data Original western blot images This paper Mendeley Data: https://doi.org/ 10.17632/nw7sbsh56p.1 Raw and processed bulk RNA-sequencing data This paper GEO: GSE254103 Raw and processed single-nuclei RNA-sequencing data This paper GEO: GSE254104 Raw and processed proteomics data This paper PRIDE: PXD053544 Experimental models: Organisms/strains Prnp-MAPT*P301S (B6/C3) The Jackson Laboratory RRID:IMSR_JAX:008169 C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:000664 Human APOE3 or APOE4 (C57BL/6J) Sullivan et al.,55 Duke University N/A Human TREM2-CV (C57BL/6J) This paper N/A Human TREM2-R47H (C57BL/6J) Gan et al.,34 Weill Cornell Medicine N/A Software and algorithms Original code This paper Zenodo: https://doi.org/10.5281/ zenodo.13642999 R 4.2.3 The R project https://www.r-project.org/ (Continued on next page) e2 Neuron 112, 1–20.e1–e8, December 4, 2024

    Protease Inhibitor:

    Article Title: Alzheimer's disease-linked risk alleles elevate microglial cGAS-associated senescence and neurodegeneration in a tauopathy model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Sudan black B, fat soluble diazo dye Abcam Cat. # ab146284 Trypsin Gibco Cat. # 15090-45 DNase I Millipore Sigma Cat. # 260913 DMEM Corning Cat. # 10-017-CV Fetal bovine serum Gibco Cat. # 26140-079 Penicillin-streptomycin Gibco Cat. # 15070063 Poly-D-lysine Thermo Fisher Scientific Cat. # A389401 Recombinant mouse GM-CSF, carrier free R&D Systems Cat. # 415-ML-020-CF 0N4R tau fibrils Sue-Ann Mok, University of Alberta N/A RIPA lysis and extraction buffer Thermo Fisher Scientific Cat. # 89901 Halt phosphatase inhibitor cocktail Thermo Fisher Scientific Cat. # 78420 cOmplete protease inhibitor cocktail Roche Cat. # 11697498001 NuPAGE MES SDS running buffer Invitrogen Cat. # NP0002 Clarity Western ECL substrate Bio-Rad Cat. # 1705060 Calcein AM Invitrogen Cat. # C3099 Etoposide Sigma-Aldrich E1383 BAI1 (BAX channel blocker) Sigma-Aldrich96 Cat. # 196805 TDI6570 (cGAS inhibitor) Synthesized by Gan Lab at Wuxi AppTec (Tianjin) Co., Ltd.97 N/A Dimethyl sulfoxide (DMSO) MP Biomedicals Cat. # 02196055 Critical commercial assays PureLink RNA Mini Kit Invitrogen Cat. # 12183018A Nuclei Isolation Kit: Nuclei PURE Prep Sigma-Aldrich Cat. # NUC201-1KT Chromium Single Cell 30 Reagent Kit 10x Genomics Cat. # PN-1000268 MOM Basic Immunodetection Kit Vector Laboratories Cat. # BMK-2202 Quick-RNA MicroPrep Kit Zymo Research Cat. # R1051 BCA protein assay kit Pierce Cat. # 23225 ProcartaPlex Mouse Chemokine Panel 1, 9plex kit Themo Fisher Scientific Cat. # EPX090-20821-901 Mouse IFN-beta ELISA kit, high sensitivity PBL Assay Science Cat. # 42410 BrdU cell proliferation ELISA kit Abcam Cat. # ab126556 Deposited data Original western blot images This paper Mendeley Data: https://doi.org/ 10.17632/nw7sbsh56p.1 Raw and processed bulk RNA-sequencing data This paper GEO: GSE254103 Raw and processed single-nuclei RNA-sequencing data This paper GEO: GSE254104 Raw and processed proteomics data This paper PRIDE: PXD053544 Experimental models: Organisms/strains Prnp-MAPT*P301S (B6/C3) The Jackson Laboratory RRID:IMSR_JAX:008169 C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:000664 Human APOE3 or APOE4 (C57BL/6J) Sullivan et al.,55 Duke University N/A Human TREM2-CV (C57BL/6J) This paper N/A Human TREM2-R47H (C57BL/6J) Gan et al.,34 Weill Cornell Medicine N/A Software and algorithms Original code This paper Zenodo: https://doi.org/10.5281/ zenodo.13642999 R 4.2.3 The R project https://www.r-project.org/ (Continued on next page) e2 Neuron 112, 1–20.e1–e8, December 4, 2024

    Western Blot:

    Article Title: Alzheimer's disease-linked risk alleles elevate microglial cGAS-associated senescence and neurodegeneration in a tauopathy model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Sudan black B, fat soluble diazo dye Abcam Cat. # ab146284 Trypsin Gibco Cat. # 15090-45 DNase I Millipore Sigma Cat. # 260913 DMEM Corning Cat. # 10-017-CV Fetal bovine serum Gibco Cat. # 26140-079 Penicillin-streptomycin Gibco Cat. # 15070063 Poly-D-lysine Thermo Fisher Scientific Cat. # A389401 Recombinant mouse GM-CSF, carrier free R&D Systems Cat. # 415-ML-020-CF 0N4R tau fibrils Sue-Ann Mok, University of Alberta N/A RIPA lysis and extraction buffer Thermo Fisher Scientific Cat. # 89901 Halt phosphatase inhibitor cocktail Thermo Fisher Scientific Cat. # 78420 cOmplete protease inhibitor cocktail Roche Cat. # 11697498001 NuPAGE MES SDS running buffer Invitrogen Cat. # NP0002 Clarity Western ECL substrate Bio-Rad Cat. # 1705060 Calcein AM Invitrogen Cat. # C3099 Etoposide Sigma-Aldrich E1383 BAI1 (BAX channel blocker) Sigma-Aldrich96 Cat. # 196805 TDI6570 (cGAS inhibitor) Synthesized by Gan Lab at Wuxi AppTec (Tianjin) Co., Ltd.97 N/A Dimethyl sulfoxide (DMSO) MP Biomedicals Cat. # 02196055 Critical commercial assays PureLink RNA Mini Kit Invitrogen Cat. # 12183018A Nuclei Isolation Kit: Nuclei PURE Prep Sigma-Aldrich Cat. # NUC201-1KT Chromium Single Cell 30 Reagent Kit 10x Genomics Cat. # PN-1000268 MOM Basic Immunodetection Kit Vector Laboratories Cat. # BMK-2202 Quick-RNA MicroPrep Kit Zymo Research Cat. # R1051 BCA protein assay kit Pierce Cat. # 23225 ProcartaPlex Mouse Chemokine Panel 1, 9plex kit Themo Fisher Scientific Cat. # EPX090-20821-901 Mouse IFN-beta ELISA kit, high sensitivity PBL Assay Science Cat. # 42410 BrdU cell proliferation ELISA kit Abcam Cat. # ab126556 Deposited data Original western blot images This paper Mendeley Data: https://doi.org/ 10.17632/nw7sbsh56p.1 Raw and processed bulk RNA-sequencing data This paper GEO: GSE254103 Raw and processed single-nuclei RNA-sequencing data This paper GEO: GSE254104 Raw and processed proteomics data This paper PRIDE: PXD053544 Experimental models: Organisms/strains Prnp-MAPT*P301S (B6/C3) The Jackson Laboratory RRID:IMSR_JAX:008169 C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:000664 Human APOE3 or APOE4 (C57BL/6J) Sullivan et al.,55 Duke University N/A Human TREM2-CV (C57BL/6J) This paper N/A Human TREM2-R47H (C57BL/6J) Gan et al.,34 Weill Cornell Medicine N/A Software and algorithms Original code This paper Zenodo: https://doi.org/10.5281/ zenodo.13642999 R 4.2.3 The R project https://www.r-project.org/ (Continued on next page) e2 Neuron 112, 1–20.e1–e8, December 4, 2024

    Synthesized:

    Article Title: Alzheimer's disease-linked risk alleles elevate microglial cGAS-associated senescence and neurodegeneration in a tauopathy model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Sudan black B, fat soluble diazo dye Abcam Cat. # ab146284 Trypsin Gibco Cat. # 15090-45 DNase I Millipore Sigma Cat. # 260913 DMEM Corning Cat. # 10-017-CV Fetal bovine serum Gibco Cat. # 26140-079 Penicillin-streptomycin Gibco Cat. # 15070063 Poly-D-lysine Thermo Fisher Scientific Cat. # A389401 Recombinant mouse GM-CSF, carrier free R&D Systems Cat. # 415-ML-020-CF 0N4R tau fibrils Sue-Ann Mok, University of Alberta N/A RIPA lysis and extraction buffer Thermo Fisher Scientific Cat. # 89901 Halt phosphatase inhibitor cocktail Thermo Fisher Scientific Cat. # 78420 cOmplete protease inhibitor cocktail Roche Cat. # 11697498001 NuPAGE MES SDS running buffer Invitrogen Cat. # NP0002 Clarity Western ECL substrate Bio-Rad Cat. # 1705060 Calcein AM Invitrogen Cat. # C3099 Etoposide Sigma-Aldrich E1383 BAI1 (BAX channel blocker) Sigma-Aldrich96 Cat. # 196805 TDI6570 (cGAS inhibitor) Synthesized by Gan Lab at Wuxi AppTec (Tianjin) Co., Ltd.97 N/A Dimethyl sulfoxide (DMSO) MP Biomedicals Cat. # 02196055 Critical commercial assays PureLink RNA Mini Kit Invitrogen Cat. # 12183018A Nuclei Isolation Kit: Nuclei PURE Prep Sigma-Aldrich Cat. # NUC201-1KT Chromium Single Cell 30 Reagent Kit 10x Genomics Cat. # PN-1000268 MOM Basic Immunodetection Kit Vector Laboratories Cat. # BMK-2202 Quick-RNA MicroPrep Kit Zymo Research Cat. # R1051 BCA protein assay kit Pierce Cat. # 23225 ProcartaPlex Mouse Chemokine Panel 1, 9plex kit Themo Fisher Scientific Cat. # EPX090-20821-901 Mouse IFN-beta ELISA kit, high sensitivity PBL Assay Science Cat. # 42410 BrdU cell proliferation ELISA kit Abcam Cat. # ab126556 Deposited data Original western blot images This paper Mendeley Data: https://doi.org/ 10.17632/nw7sbsh56p.1 Raw and processed bulk RNA-sequencing data This paper GEO: GSE254103 Raw and processed single-nuclei RNA-sequencing data This paper GEO: GSE254104 Raw and processed proteomics data This paper PRIDE: PXD053544 Experimental models: Organisms/strains Prnp-MAPT*P301S (B6/C3) The Jackson Laboratory RRID:IMSR_JAX:008169 C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:000664 Human APOE3 or APOE4 (C57BL/6J) Sullivan et al.,55 Duke University N/A Human TREM2-CV (C57BL/6J) This paper N/A Human TREM2-R47H (C57BL/6J) Gan et al.,34 Weill Cornell Medicine N/A Software and algorithms Original code This paper Zenodo: https://doi.org/10.5281/ zenodo.13642999 R 4.2.3 The R project https://www.r-project.org/ (Continued on next page) e2 Neuron 112, 1–20.e1–e8, December 4, 2024

    Isolation:

    Article Title: Alzheimer's disease-linked risk alleles elevate microglial cGAS-associated senescence and neurodegeneration in a tauopathy model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Sudan black B, fat soluble diazo dye Abcam Cat. # ab146284 Trypsin Gibco Cat. # 15090-45 DNase I Millipore Sigma Cat. # 260913 DMEM Corning Cat. # 10-017-CV Fetal bovine serum Gibco Cat. # 26140-079 Penicillin-streptomycin Gibco Cat. # 15070063 Poly-D-lysine Thermo Fisher Scientific Cat. # A389401 Recombinant mouse GM-CSF, carrier free R&D Systems Cat. # 415-ML-020-CF 0N4R tau fibrils Sue-Ann Mok, University of Alberta N/A RIPA lysis and extraction buffer Thermo Fisher Scientific Cat. # 89901 Halt phosphatase inhibitor cocktail Thermo Fisher Scientific Cat. # 78420 cOmplete protease inhibitor cocktail Roche Cat. # 11697498001 NuPAGE MES SDS running buffer Invitrogen Cat. # NP0002 Clarity Western ECL substrate Bio-Rad Cat. # 1705060 Calcein AM Invitrogen Cat. # C3099 Etoposide Sigma-Aldrich E1383 BAI1 (BAX channel blocker) Sigma-Aldrich96 Cat. # 196805 TDI6570 (cGAS inhibitor) Synthesized by Gan Lab at Wuxi AppTec (Tianjin) Co., Ltd.97 N/A Dimethyl sulfoxide (DMSO) MP Biomedicals Cat. # 02196055 Critical commercial assays PureLink RNA Mini Kit Invitrogen Cat. # 12183018A Nuclei Isolation Kit: Nuclei PURE Prep Sigma-Aldrich Cat. # NUC201-1KT Chromium Single Cell 30 Reagent Kit 10x Genomics Cat. # PN-1000268 MOM Basic Immunodetection Kit Vector Laboratories Cat. # BMK-2202 Quick-RNA MicroPrep Kit Zymo Research Cat. # R1051 BCA protein assay kit Pierce Cat. # 23225 ProcartaPlex Mouse Chemokine Panel 1, 9plex kit Themo Fisher Scientific Cat. # EPX090-20821-901 Mouse IFN-beta ELISA kit, high sensitivity PBL Assay Science Cat. # 42410 BrdU cell proliferation ELISA kit Abcam Cat. # ab126556 Deposited data Original western blot images This paper Mendeley Data: https://doi.org/ 10.17632/nw7sbsh56p.1 Raw and processed bulk RNA-sequencing data This paper GEO: GSE254103 Raw and processed single-nuclei RNA-sequencing data This paper GEO: GSE254104 Raw and processed proteomics data This paper PRIDE: PXD053544 Experimental models: Organisms/strains Prnp-MAPT*P301S (B6/C3) The Jackson Laboratory RRID:IMSR_JAX:008169 C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:000664 Human APOE3 or APOE4 (C57BL/6J) Sullivan et al.,55 Duke University N/A Human TREM2-CV (C57BL/6J) This paper N/A Human TREM2-R47H (C57BL/6J) Gan et al.,34 Weill Cornell Medicine N/A Software and algorithms Original code This paper Zenodo: https://doi.org/10.5281/ zenodo.13642999 R 4.2.3 The R project https://www.r-project.org/ (Continued on next page) e2 Neuron 112, 1–20.e1–e8, December 4, 2024

    Immunodetection:

    Article Title: Alzheimer's disease-linked risk alleles elevate microglial cGAS-associated senescence and neurodegeneration in a tauopathy model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Sudan black B, fat soluble diazo dye Abcam Cat. # ab146284 Trypsin Gibco Cat. # 15090-45 DNase I Millipore Sigma Cat. # 260913 DMEM Corning Cat. # 10-017-CV Fetal bovine serum Gibco Cat. # 26140-079 Penicillin-streptomycin Gibco Cat. # 15070063 Poly-D-lysine Thermo Fisher Scientific Cat. # A389401 Recombinant mouse GM-CSF, carrier free R&D Systems Cat. # 415-ML-020-CF 0N4R tau fibrils Sue-Ann Mok, University of Alberta N/A RIPA lysis and extraction buffer Thermo Fisher Scientific Cat. # 89901 Halt phosphatase inhibitor cocktail Thermo Fisher Scientific Cat. # 78420 cOmplete protease inhibitor cocktail Roche Cat. # 11697498001 NuPAGE MES SDS running buffer Invitrogen Cat. # NP0002 Clarity Western ECL substrate Bio-Rad Cat. # 1705060 Calcein AM Invitrogen Cat. # C3099 Etoposide Sigma-Aldrich E1383 BAI1 (BAX channel blocker) Sigma-Aldrich96 Cat. # 196805 TDI6570 (cGAS inhibitor) Synthesized by Gan Lab at Wuxi AppTec (Tianjin) Co., Ltd.97 N/A Dimethyl sulfoxide (DMSO) MP Biomedicals Cat. # 02196055 Critical commercial assays PureLink RNA Mini Kit Invitrogen Cat. # 12183018A Nuclei Isolation Kit: Nuclei PURE Prep Sigma-Aldrich Cat. # NUC201-1KT Chromium Single Cell 30 Reagent Kit 10x Genomics Cat. # PN-1000268 MOM Basic Immunodetection Kit Vector Laboratories Cat. # BMK-2202 Quick-RNA MicroPrep Kit Zymo Research Cat. # R1051 BCA protein assay kit Pierce Cat. # 23225 ProcartaPlex Mouse Chemokine Panel 1, 9plex kit Themo Fisher Scientific Cat. # EPX090-20821-901 Mouse IFN-beta ELISA kit, high sensitivity PBL Assay Science Cat. # 42410 BrdU cell proliferation ELISA kit Abcam Cat. # ab126556 Deposited data Original western blot images This paper Mendeley Data: https://doi.org/ 10.17632/nw7sbsh56p.1 Raw and processed bulk RNA-sequencing data This paper GEO: GSE254103 Raw and processed single-nuclei RNA-sequencing data This paper GEO: GSE254104 Raw and processed proteomics data This paper PRIDE: PXD053544 Experimental models: Organisms/strains Prnp-MAPT*P301S (B6/C3) The Jackson Laboratory RRID:IMSR_JAX:008169 C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:000664 Human APOE3 or APOE4 (C57BL/6J) Sullivan et al.,55 Duke University N/A Human TREM2-CV (C57BL/6J) This paper N/A Human TREM2-R47H (C57BL/6J) Gan et al.,34 Weill Cornell Medicine N/A Software and algorithms Original code This paper Zenodo: https://doi.org/10.5281/ zenodo.13642999 R 4.2.3 The R project https://www.r-project.org/ (Continued on next page) e2 Neuron 112, 1–20.e1–e8, December 4, 2024

    Bicinchoninic Acid Protein Assay:

    Article Title: Alzheimer's disease-linked risk alleles elevate microglial cGAS-associated senescence and neurodegeneration in a tauopathy model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Sudan black B, fat soluble diazo dye Abcam Cat. # ab146284 Trypsin Gibco Cat. # 15090-45 DNase I Millipore Sigma Cat. # 260913 DMEM Corning Cat. # 10-017-CV Fetal bovine serum Gibco Cat. # 26140-079 Penicillin-streptomycin Gibco Cat. # 15070063 Poly-D-lysine Thermo Fisher Scientific Cat. # A389401 Recombinant mouse GM-CSF, carrier free R&D Systems Cat. # 415-ML-020-CF 0N4R tau fibrils Sue-Ann Mok, University of Alberta N/A RIPA lysis and extraction buffer Thermo Fisher Scientific Cat. # 89901 Halt phosphatase inhibitor cocktail Thermo Fisher Scientific Cat. # 78420 cOmplete protease inhibitor cocktail Roche Cat. # 11697498001 NuPAGE MES SDS running buffer Invitrogen Cat. # NP0002 Clarity Western ECL substrate Bio-Rad Cat. # 1705060 Calcein AM Invitrogen Cat. # C3099 Etoposide Sigma-Aldrich E1383 BAI1 (BAX channel blocker) Sigma-Aldrich96 Cat. # 196805 TDI6570 (cGAS inhibitor) Synthesized by Gan Lab at Wuxi AppTec (Tianjin) Co., Ltd.97 N/A Dimethyl sulfoxide (DMSO) MP Biomedicals Cat. # 02196055 Critical commercial assays PureLink RNA Mini Kit Invitrogen Cat. # 12183018A Nuclei Isolation Kit: Nuclei PURE Prep Sigma-Aldrich Cat. # NUC201-1KT Chromium Single Cell 30 Reagent Kit 10x Genomics Cat. # PN-1000268 MOM Basic Immunodetection Kit Vector Laboratories Cat. # BMK-2202 Quick-RNA MicroPrep Kit Zymo Research Cat. # R1051 BCA protein assay kit Pierce Cat. # 23225 ProcartaPlex Mouse Chemokine Panel 1, 9plex kit Themo Fisher Scientific Cat. # EPX090-20821-901 Mouse IFN-beta ELISA kit, high sensitivity PBL Assay Science Cat. # 42410 BrdU cell proliferation ELISA kit Abcam Cat. # ab126556 Deposited data Original western blot images This paper Mendeley Data: https://doi.org/ 10.17632/nw7sbsh56p.1 Raw and processed bulk RNA-sequencing data This paper GEO: GSE254103 Raw and processed single-nuclei RNA-sequencing data This paper GEO: GSE254104 Raw and processed proteomics data This paper PRIDE: PXD053544 Experimental models: Organisms/strains Prnp-MAPT*P301S (B6/C3) The Jackson Laboratory RRID:IMSR_JAX:008169 C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:000664 Human APOE3 or APOE4 (C57BL/6J) Sullivan et al.,55 Duke University N/A Human TREM2-CV (C57BL/6J) This paper N/A Human TREM2-R47H (C57BL/6J) Gan et al.,34 Weill Cornell Medicine N/A Software and algorithms Original code This paper Zenodo: https://doi.org/10.5281/ zenodo.13642999 R 4.2.3 The R project https://www.r-project.org/ (Continued on next page) e2 Neuron 112, 1–20.e1–e8, December 4, 2024

    Enzyme-linked Immunosorbent Assay:

    Article Title: Alzheimer's disease-linked risk alleles elevate microglial cGAS-associated senescence and neurodegeneration in a tauopathy model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Sudan black B, fat soluble diazo dye Abcam Cat. # ab146284 Trypsin Gibco Cat. # 15090-45 DNase I Millipore Sigma Cat. # 260913 DMEM Corning Cat. # 10-017-CV Fetal bovine serum Gibco Cat. # 26140-079 Penicillin-streptomycin Gibco Cat. # 15070063 Poly-D-lysine Thermo Fisher Scientific Cat. # A389401 Recombinant mouse GM-CSF, carrier free R&D Systems Cat. # 415-ML-020-CF 0N4R tau fibrils Sue-Ann Mok, University of Alberta N/A RIPA lysis and extraction buffer Thermo Fisher Scientific Cat. # 89901 Halt phosphatase inhibitor cocktail Thermo Fisher Scientific Cat. # 78420 cOmplete protease inhibitor cocktail Roche Cat. # 11697498001 NuPAGE MES SDS running buffer Invitrogen Cat. # NP0002 Clarity Western ECL substrate Bio-Rad Cat. # 1705060 Calcein AM Invitrogen Cat. # C3099 Etoposide Sigma-Aldrich E1383 BAI1 (BAX channel blocker) Sigma-Aldrich96 Cat. # 196805 TDI6570 (cGAS inhibitor) Synthesized by Gan Lab at Wuxi AppTec (Tianjin) Co., Ltd.97 N/A Dimethyl sulfoxide (DMSO) MP Biomedicals Cat. # 02196055 Critical commercial assays PureLink RNA Mini Kit Invitrogen Cat. # 12183018A Nuclei Isolation Kit: Nuclei PURE Prep Sigma-Aldrich Cat. # NUC201-1KT Chromium Single Cell 30 Reagent Kit 10x Genomics Cat. # PN-1000268 MOM Basic Immunodetection Kit Vector Laboratories Cat. # BMK-2202 Quick-RNA MicroPrep Kit Zymo Research Cat. # R1051 BCA protein assay kit Pierce Cat. # 23225 ProcartaPlex Mouse Chemokine Panel 1, 9plex kit Themo Fisher Scientific Cat. # EPX090-20821-901 Mouse IFN-beta ELISA kit, high sensitivity PBL Assay Science Cat. # 42410 BrdU cell proliferation ELISA kit Abcam Cat. # ab126556 Deposited data Original western blot images This paper Mendeley Data: https://doi.org/ 10.17632/nw7sbsh56p.1 Raw and processed bulk RNA-sequencing data This paper GEO: GSE254103 Raw and processed single-nuclei RNA-sequencing data This paper GEO: GSE254104 Raw and processed proteomics data This paper PRIDE: PXD053544 Experimental models: Organisms/strains Prnp-MAPT*P301S (B6/C3) The Jackson Laboratory RRID:IMSR_JAX:008169 C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:000664 Human APOE3 or APOE4 (C57BL/6J) Sullivan et al.,55 Duke University N/A Human TREM2-CV (C57BL/6J) This paper N/A Human TREM2-R47H (C57BL/6J) Gan et al.,34 Weill Cornell Medicine N/A Software and algorithms Original code This paper Zenodo: https://doi.org/10.5281/ zenodo.13642999 R 4.2.3 The R project https://www.r-project.org/ (Continued on next page) e2 Neuron 112, 1–20.e1–e8, December 4, 2024

    Software:

    Article Title: Alzheimer's disease-linked risk alleles elevate microglial cGAS-associated senescence and neurodegeneration in a tauopathy model.
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Sudan black B, fat soluble diazo dye Abcam Cat. # ab146284 Trypsin Gibco Cat. # 15090-45 DNase I Millipore Sigma Cat. # 260913 DMEM Corning Cat. # 10-017-CV Fetal bovine serum Gibco Cat. # 26140-079 Penicillin-streptomycin Gibco Cat. # 15070063 Poly-D-lysine Thermo Fisher Scientific Cat. # A389401 Recombinant mouse GM-CSF, carrier free R&D Systems Cat. # 415-ML-020-CF 0N4R tau fibrils Sue-Ann Mok, University of Alberta N/A RIPA lysis and extraction buffer Thermo Fisher Scientific Cat. # 89901 Halt phosphatase inhibitor cocktail Thermo Fisher Scientific Cat. # 78420 cOmplete protease inhibitor cocktail Roche Cat. # 11697498001 NuPAGE MES SDS running buffer Invitrogen Cat. # NP0002 Clarity Western ECL substrate Bio-Rad Cat. # 1705060 Calcein AM Invitrogen Cat. # C3099 Etoposide Sigma-Aldrich E1383 BAI1 (BAX channel blocker) Sigma-Aldrich96 Cat. # 196805 TDI6570 (cGAS inhibitor) Synthesized by Gan Lab at Wuxi AppTec (Tianjin) Co., Ltd.97 N/A Dimethyl sulfoxide (DMSO) MP Biomedicals Cat. # 02196055 Critical commercial assays PureLink RNA Mini Kit Invitrogen Cat. # 12183018A Nuclei Isolation Kit: Nuclei PURE Prep Sigma-Aldrich Cat. # NUC201-1KT Chromium Single Cell 30 Reagent Kit 10x Genomics Cat. # PN-1000268 MOM Basic Immunodetection Kit Vector Laboratories Cat. # BMK-2202 Quick-RNA MicroPrep Kit Zymo Research Cat. # R1051 BCA protein assay kit Pierce Cat. # 23225 ProcartaPlex Mouse Chemokine Panel 1, 9plex kit Themo Fisher Scientific Cat. # EPX090-20821-901 Mouse IFN-beta ELISA kit, high sensitivity PBL Assay Science Cat. # 42410 BrdU cell proliferation ELISA kit Abcam Cat. # ab126556 Deposited data Original western blot images This paper Mendeley Data: https://doi.org/ 10.17632/nw7sbsh56p.1 Raw and processed bulk RNA-sequencing data This paper GEO: GSE254103 Raw and processed single-nuclei RNA-sequencing data This paper GEO: GSE254104 Raw and processed proteomics data This paper PRIDE: PXD053544 Experimental models: Organisms/strains Prnp-MAPT*P301S (B6/C3) The Jackson Laboratory RRID:IMSR_JAX:008169 C57BL/6J The Jackson Laboratory RRID:IMSR_JAX:000664 Human APOE3 or APOE4 (C57BL/6J) Sullivan et al.,55 Duke University N/A Human TREM2-CV (C57BL/6J) This paper N/A Human TREM2-R47H (C57BL/6J) Gan et al.,34 Weill Cornell Medicine N/A Software and algorithms Original code This paper Zenodo: https://doi.org/10.5281/ zenodo.13642999 R 4.2.3 The R project https://www.r-project.org/ (Continued on next page) e2 Neuron 112, 1–20.e1–e8, December 4, 2024



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